Direct Visualization of Trans-Synaptic Neurexin-Neuroligin Interactions during Synapse Formation
نویسندگان
چکیده
منابع مشابه
Retraction Notice to: Imaging Activity-Dependent Regulation of Neurexin-Neuroligin Interactions Using trans-Synaptic Enzymatic Biotinylation
(Cell 143, 456–469; October 7, 2010) This paper introduced a new methodology, BLINC, for detecting the trans-synaptic binding of neurexin and neuroligin proteins, and applied BLINC to study the interaction dynamics of these proteins in neurons. Since this publication, my laboratory has found that BLINC cannot be reproduced in neurons using the constructs and protocols described in this paper. A...
متن کاملAlternative Splicing Controls Selective Trans-Synaptic Interactions of the Neuroligin-Neurexin Complex
Formation of synapses requires specific cellular interactions that organize pre- and postsynaptic compartments. The neuroligin-neurexin complex mediates heterophilic adhesion and can trigger assembly of glutamatergic and GABAergic synapses in cultured hippocampal neurons. Both neuroligins and neurexins are encoded by multiple genes. Alternative splicing generates large numbers of isoforms, whic...
متن کاملRETRACTED: Imaging Activity-Dependent Regulation of Neurexin-Neuroligin Interactions Using trans-Synaptic Enzymatic Biotinylation
The functions of trans-synaptic adhesion molecules, such as neurexin and neuroligin, have been difficult to study due to the lack of methods to directly detect their binding in living neurons. Here, we use biotin labeling of intercellular contacts (BLINC), a method for imaging protein interactions based on interaction-dependent biotinylation of a peptide by E. coli biotin ligase, to visualize n...
متن کاملImaging Trans-Cellular Neurexin-Neuroligin Interactions by Enzymatic Probe Ligation
Neurexin and neuroligin are transmembrane adhesion proteins that play an important role in organizing the neuronal synaptic cleft. Our lab previously reported a method for imaging the trans-synaptic binding of neurexin and neuroligin called BLINC (Biotin Labeling of INtercellular Contacts). In BLINC, biotin ligase (BirA) is fused to one protein while its 15-amino acid acceptor peptide substrate...
متن کاملNeurexin-neuroligin signaling in synapse development.
Neurexins and neuroligins are emerging as central organizing molecules for excitatory glutamatergic and inhibitory GABAergic synapses in mammalian brain. They function as cell adhesion molecules, bridging the synaptic cleft. Remarkably, each partner can trigger formation of a hemisynapse: neuroligins trigger presynaptic differentiation and neurexins trigger postsynaptic differentiation. Recent ...
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ژورنال
عنوان ژورنال: Journal of Neuroscience
سال: 2014
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.0348-14.2014